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mac2 primary antibody  (Cedarlane)


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    Structured Review

    Cedarlane mac2 primary antibody
    Mac2 Primary Antibody, supplied by Cedarlane, used in various techniques. Bioz Stars score: 96/100, based on 398 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/primary+rat+antibody+anti-mac2+cl8942ap/Anti-Mouse%2FHuman+Mac-2+(Galectin-3)%2C+Purified+(Clone+M3%2F38)+(rat+IgG2a)/pmc10723050__pnas__2314698120__sapp-110-10-13
    Average 96 stars, based on 398 article reviews
    mac2 primary antibody - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Incubation:

    Article Title: HIIT Promotes M2 Macrophage Polarization and Sympathetic Nerve Density to Induce Adipose Tissue Browning in T2DM Mice
    Article Snippet: .. The sections were incubated in 5% bovine serum albumin for 2 h, followed by overnight incubation at 4 °C with the following primary antibodies: rat anti-Mac2 antibody (1:500, Cedarlane, Burlington, ON, Canada, CL8942AP), rabbit anti-iNOS antibody (1:500, Abcam, Cambridge, UK, ab15323), rabbit anti-Arg1 antibody (1:500, Cell Signaling Technology, Boston, MA, USA, 93668), and sheep anti-TH antibody (1:500, Novus Biologicals, CO, USA, NB300-110). .. Next, the sections were incubated with the following secondary antibodies: Alexa 555-Donkey Anti-Rat IgG (1:1000, Abcam, ab150154), Alexa 488-Donkey Anti-Rabbit IgG (1:1000, Abcam, ab150073), and Alexa 647-Donkey Anti-sheep IgG (1:1000, Abcam, ab150179).

    Article Title: Supporting Information for Distinct mouse models of Stargardt disease display differences in inflammatory responses and pharmacological targeting of ceramides
    Article Snippet: Membranes were briefly washed in PBS supplemented with 0.05% Tween-20 (Sigma Aldrich, St. Louis, MO, #9416) volume/volume (1x PBS-t), before incubation with 5% milk (dry mass/volume) in 1x PBS-t for 1 hr at room temperature. .. The 5% milk was then removed and replaced with rat-anti-mouse Mac2 primary antibody (CedarLane Labs, Burlington NC, #CL8942AP) at a 1:1000 dilution and rabbit-anti-mouse a-tubulin primary antibody at a 1:2000 dilution in 1% milk in PBS-t. Membranes were bathed with primary antibodies overnight on a rocking platform at 4°C before washing with PBS-t, and incubation with peroxidase-conjugated anti-rat secondary antibody (Jackson ImmunoResearch Labs, West Grove PA #112035167) at a 1:1000 dilution and peroxidase conjugated anti-rabbit secondary antibody (Cell Signaling Technology USA, Danvers MA #7074S) at a 1:2000 dilution in PBS-t at room temperature for 1 hr. .. Membranes were then washed with 1x PBS-t and incubated for approximately 30 sec with SuperSignal West Femto Maximum Sensitivity Substrate (ThermoFisher Scientific, Waltham, MA, #PI34094), before the membranes were placed between two clean plastic sheets and imaged, using the same exposure time for each membrane.

    Article Title: Protective Effect of Mesenchymal Stromal Cells in Diabetic Nephropathy: The <i>In vitro </i>and <i>In vivo</i> Role of the M-Sec-Tunneling Nanotubes.
    Article Snippet: Federica Barutta1, Beatrice Corbetta1, Stefania Bellini1, Roberto Gambino1, Stefania Bruno1, Shunsuke Kimura2, Koji Hase2, Hiroshi Ohno3 and Gabriella Gruden1 1Department of Medical Sciences, University of Turin, Turin, Italy; 2Division of Biochemistry, Faculty of Pharmacy, Keio University, Tokyo, Japan; 3Laboratory for Intestinal Ecosystem, RIKEN Center for Integrative Medical Sciences, Yokohama, Japan

    Article Title: Protective effect of mesenchymal stromal cells in diabetic nephropathy: the In vitro and In vivo role of the M-Sec-tunneling nanotubes
    Article Snippet: .. Subsequently, the slides were incubated with 3% BSA in phosphate buffer saline (PBS) for 30 min. Overnight incubation at 4°C was performed with a primary rat antibody anti-MAC2 (Cedarlane; CL8942AP). .. After washing with PBS, sections were exposed to a secondary biotinylated goat anti-rat antibody (Jackson ImmunoResearch Laboratories; 112–065-003) for 1 h, followed by incubation with horseradish peroxidase (HRP)-conjugated streptavidin (DAKO, P0397) for 1 h. Finally, sections were mounted and 3,3′- diaminobenzidine (DAB) used as the chromogen substrate for HRP.

    Article Title: Cardiomyocyte-Restricted Expression of IL11 Causes Cardiac Fibrosis, Inflammation, and Dysfunction.
    Article Snippet: 2023, 24, 12989 11 of 14 Santa Clara, CA, USA, S2023) and serum-free blocker (Leica Biosystems, Deer Park, IL, USA, RE7102.) .. The sections were then incubated with primary antibody against MAC2 (Cedarlane, Burlington, ON, Canada, CL8942AP) overnight at 4 ◦C and secondary antirat (Vector, Stuttgart, Germany, BA-4001) for 1 h. The staining was visualized using a NovoLink polymer detection system (Leica Biosystems: RE7112, RE7105, RE7143). .. The sections were then counterstained with hematoxylin and imaged using a Zeiss Axio scan digital slide scanner.

    Article Title: Cardiomyocyte-Restricted Expression of IL11 Causes Cardiac Fibrosis, Inflammation, and Dysfunction
    Article Snippet: The slides were blocked in DAKO peroxidase blocking solution (Aglient Techniologies, Santa Clara, CA, USA, S2023) and serum-free blocker (Leica Biosystems, Deer Park, IL, USA, RE7102.) .. The sections were then incubated with primary antibody against MAC2 (Cedarlane, Burlington, ON, Canada, CL8942AP) overnight at 4 °C and secondary anti-rat (Vector, Stuttgart, Germany, BA-4001) for 1 h. The staining was visualized using a NovoLink polymer detection system (Leica Biosystems: RE7112, RE7105, RE7143). .. The sections were then counterstained with hematoxylin and imaged using a Zeiss Axio scan digital slide scanner.

    Article Title: Macrophage-to-endothelial cell crosstalk by the cholesterol metabolite 27HC promotes atherosclerosis in male mice.
    Article Snippet: .. Macrophage abundance and adhesion molecule expression were assessed by blocking in buffer containing 10% donkey serum and 5% BAS for 1 h at room temperature following antigen retrieval with citric acid (pH6.0), and incubation at 4 °C overnight with the following primary antibodies: Mac2 (Cedarlane, CL8942AP, 1:600), ICAM-1 (R&D, AF796, 1:400), VCAM-1 (R&D, AF643, 1:400), and CD31 (Abcam, ab28364, 1:500). .. The secondary antibodies were donkey anti-rat conjugated to Alexa 488 (Thermo Fisher Scientific, A-21208, 1:600), donkey anti-goat conjugated to Alexa 647 (Thermo Fisher Scientific, A21447, 1:600), and donkey anti-rabbit conjugated to Alexa 488 (Thermo Fisher Scientific, A-21206, 1:600).

    Saline:

    Article Title: Protective Effect of Mesenchymal Stromal Cells in Diabetic Nephropathy: The <i>In vitro </i>and <i>In vivo</i> Role of the M-Sec-Tunneling Nanotubes.
    Article Snippet: Federica Barutta1, Beatrice Corbetta1, Stefania Bellini1, Roberto Gambino1, Stefania Bruno1, Shunsuke Kimura2, Koji Hase2, Hiroshi Ohno3 and Gabriella Gruden1 1Department of Medical Sciences, University of Turin, Turin, Italy; 2Division of Biochemistry, Faculty of Pharmacy, Keio University, Tokyo, Japan; 3Laboratory for Intestinal Ecosystem, RIKEN Center for Integrative Medical Sciences, Yokohama, Japan

    Article Title: Protective effect of mesenchymal stromal cells in diabetic nephropathy: the In vitro and In vivo role of the M-Sec-tunneling nanotubes
    Article Snippet: .. Subsequently, the slides were incubated with 3% BSA in phosphate buffer saline (PBS) for 30 min. Overnight incubation at 4°C was performed with a primary rat antibody anti-MAC2 (Cedarlane; CL8942AP). .. After washing with PBS, sections were exposed to a secondary biotinylated goat anti-rat antibody (Jackson ImmunoResearch Laboratories; 112–065-003) for 1 h, followed by incubation with horseradish peroxidase (HRP)-conjugated streptavidin (DAKO, P0397) for 1 h. Finally, sections were mounted and 3,3′- diaminobenzidine (DAB) used as the chromogen substrate for HRP.

    Staining:

    Article Title: Cardiomyocyte-Restricted Expression of IL11 Causes Cardiac Fibrosis, Inflammation, and Dysfunction.
    Article Snippet: 2023, 24, 12989 11 of 14 Santa Clara, CA, USA, S2023) and serum-free blocker (Leica Biosystems, Deer Park, IL, USA, RE7102.) .. The sections were then incubated with primary antibody against MAC2 (Cedarlane, Burlington, ON, Canada, CL8942AP) overnight at 4 ◦C and secondary antirat (Vector, Stuttgart, Germany, BA-4001) for 1 h. The staining was visualized using a NovoLink polymer detection system (Leica Biosystems: RE7112, RE7105, RE7143). .. The sections were then counterstained with hematoxylin and imaged using a Zeiss Axio scan digital slide scanner.

    Article Title: <scp>CXCL13</scp> promote angiogenesis by recruiting <scp>M2</scp> macrophages and endothelial progenitor cells in beige adipose grafts
    Article Snippet: Sai Luo and Ying Liu contributed equally to this study and should be considered cocorresponding authors.. Abbreviations: ASCs, Adiposederived stem cells; BeigeAT, Beige adipose tissue; CXCL13, CXC motif chemokine ligand 13; EPCs, endothelial progenitor cells; WAT, white adipose tissue.. Correspondence Ying Liu, Plastic and Reconstructive Surgery Department of Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200001, China.

    Article Title: Cardiomyocyte-Restricted Expression of IL11 Causes Cardiac Fibrosis, Inflammation, and Dysfunction
    Article Snippet: The slides were blocked in DAKO peroxidase blocking solution (Aglient Techniologies, Santa Clara, CA, USA, S2023) and serum-free blocker (Leica Biosystems, Deer Park, IL, USA, RE7102.) .. The sections were then incubated with primary antibody against MAC2 (Cedarlane, Burlington, ON, Canada, CL8942AP) overnight at 4 °C and secondary anti-rat (Vector, Stuttgart, Germany, BA-4001) for 1 h. The staining was visualized using a NovoLink polymer detection system (Leica Biosystems: RE7112, RE7105, RE7143). .. The sections were then counterstained with hematoxylin and imaged using a Zeiss Axio scan digital slide scanner.

    Polymer:

    Article Title: Cardiomyocyte-Restricted Expression of IL11 Causes Cardiac Fibrosis, Inflammation, and Dysfunction.
    Article Snippet: 2023, 24, 12989 11 of 14 Santa Clara, CA, USA, S2023) and serum-free blocker (Leica Biosystems, Deer Park, IL, USA, RE7102.) .. The sections were then incubated with primary antibody against MAC2 (Cedarlane, Burlington, ON, Canada, CL8942AP) overnight at 4 ◦C and secondary antirat (Vector, Stuttgart, Germany, BA-4001) for 1 h. The staining was visualized using a NovoLink polymer detection system (Leica Biosystems: RE7112, RE7105, RE7143). .. The sections were then counterstained with hematoxylin and imaged using a Zeiss Axio scan digital slide scanner.

    Article Title: Cardiomyocyte-Restricted Expression of IL11 Causes Cardiac Fibrosis, Inflammation, and Dysfunction
    Article Snippet: The slides were blocked in DAKO peroxidase blocking solution (Aglient Techniologies, Santa Clara, CA, USA, S2023) and serum-free blocker (Leica Biosystems, Deer Park, IL, USA, RE7102.) .. The sections were then incubated with primary antibody against MAC2 (Cedarlane, Burlington, ON, Canada, CL8942AP) overnight at 4 °C and secondary anti-rat (Vector, Stuttgart, Germany, BA-4001) for 1 h. The staining was visualized using a NovoLink polymer detection system (Leica Biosystems: RE7112, RE7105, RE7143). .. The sections were then counterstained with hematoxylin and imaged using a Zeiss Axio scan digital slide scanner.

    Immunofluorescence:

    Article Title: <scp>CXCL13</scp> promote angiogenesis by recruiting <scp>M2</scp> macrophages and endothelial progenitor cells in beige adipose grafts
    Article Snippet: Sai Luo and Ying Liu contributed equally to this study and should be considered cocorresponding authors.. Abbreviations: ASCs, Adiposederived stem cells; BeigeAT, Beige adipose tissue; CXCL13, CXC motif chemokine ligand 13; EPCs, endothelial progenitor cells; WAT, white adipose tissue.. Correspondence Ying Liu, Plastic and Reconstructive Surgery Department of Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200001, China.

    Expressing:

    Article Title: Macrophage-to-endothelial cell crosstalk by the cholesterol metabolite 27HC promotes atherosclerosis in male mice.
    Article Snippet: .. Macrophage abundance and adhesion molecule expression were assessed by blocking in buffer containing 10% donkey serum and 5% BAS for 1 h at room temperature following antigen retrieval with citric acid (pH6.0), and incubation at 4 °C overnight with the following primary antibodies: Mac2 (Cedarlane, CL8942AP, 1:600), ICAM-1 (R&D, AF796, 1:400), VCAM-1 (R&D, AF643, 1:400), and CD31 (Abcam, ab28364, 1:500). .. The secondary antibodies were donkey anti-rat conjugated to Alexa 488 (Thermo Fisher Scientific, A-21208, 1:600), donkey anti-goat conjugated to Alexa 647 (Thermo Fisher Scientific, A21447, 1:600), and donkey anti-rabbit conjugated to Alexa 488 (Thermo Fisher Scientific, A-21206, 1:600).

    Blocking Assay:

    Article Title: Macrophage-to-endothelial cell crosstalk by the cholesterol metabolite 27HC promotes atherosclerosis in male mice.
    Article Snippet: .. Macrophage abundance and adhesion molecule expression were assessed by blocking in buffer containing 10% donkey serum and 5% BAS for 1 h at room temperature following antigen retrieval with citric acid (pH6.0), and incubation at 4 °C overnight with the following primary antibodies: Mac2 (Cedarlane, CL8942AP, 1:600), ICAM-1 (R&D, AF796, 1:400), VCAM-1 (R&D, AF643, 1:400), and CD31 (Abcam, ab28364, 1:500). .. The secondary antibodies were donkey anti-rat conjugated to Alexa 488 (Thermo Fisher Scientific, A-21208, 1:600), donkey anti-goat conjugated to Alexa 647 (Thermo Fisher Scientific, A21447, 1:600), and donkey anti-rabbit conjugated to Alexa 488 (Thermo Fisher Scientific, A-21206, 1:600).



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